Synthesis, characterization, biological activity and dna. Due to cationic character, threedimensional structural profiles, and propensity for performing hydrolysis, redox, or photoreactions of metal ions and complexes, have a natural ability for interacting with dna. The dna cleavage studies of metal complexes showed more prominent activity in the presence of h 2 o 2 compared to that in the other metal complexes. The metal complexes were able to convert super coiled dna into open circular dna. Pdf electrochemical investigation and dnabinding studies. Dna binding, cleavage activity, molecular docking, cytotoxicity and genotoxicity studies of newly synthesized copper based metal complexes ayaz mahmood dar1,2, meraj alam khan1, shafia mir2. Synthesis, characterisation and dna binding studies of metal. The compounds were subjected to antimicrobial, antioxidant and antitubercular activity screening. Hence the present study aims for the synthesis, characterization, antimicrobial and dna binding studies of manganese and zinc complexes containing mixed ligands 8. Synthesis, characterization and antimicrobial screening. Aug 26, 2009 the binding properties of the two complexes to calf thymusdna ctdna were investigated by different spectrophotometric methods and viscosity measurements together with equilibrium dialysis and circular dichroism spectroscopy. Studies on dna binding to metal complexes of sal 2trien zhou chengyong1,2, xi xiaoli 1, and yang pin1 1key laboratory of chemical biology and molecular engineering of the ministry of education, institute of molecular science, shanxi university, taiyuan, shanxi 030006, pr china. A theoretical treatment of the formation of complexes in the gas phase was studied.
These complexes are lowenergy mlct absorbers and bind to dna rapidly. The ratio of absorbance at 260 and 280 nm lies within the range of 1. Oct 25, 2019 further the compounds were screened for their antimicrobial potency against few strains of bacteria and fungus and fair results were found from the above studies. Thermal analysis confirms there are no coordinated water molecules present in the metal complexes. Using fret melting assays, the binding affinity and selectivity of the five metal complexes against six different g4 dna structures as well as a duplex dna. The cytotoxicity studies of 1 and 2 complexes were discussed in this paper. Pdf dna binding, amelioration of oxidative stress, and. The three complexes and the ligand imidazole display dna binding ability, ascertained by uvvis titration. Jayabalakrishnan2 1department of chemistry, kumaraguru college of technology, coimbatore, tn, india 2department of chemistry, sri ramakrishna mission vidyalaya college of arts and science, coimbatore,tn, india. Pdf the interaction of ligands and their transition metal complexes with plasmid. The binding poses of the metal complexes 1 a and 2 b into the dna duplex obtained from molecular docking analysis.
Binding studies of metalsalphen and metalbipyridine. Design, syntheses and functional studies of dna binding metal complexes final seminar april 10, 1998 antitumor activity of many anticancer drugs is based on their interaction with dna five basic interaction types are observed. From the spectral studies an octahedral geometry was proposed for the copper complex. Metal complexes containing aromatic chelators are of great. Design, synthesis, mtt assay, dna interaction studies of. Synthesis and dna binding studies of niii, coii, cuii and znii metal complexes of n1,n5bispyridine2methylenethiocarbohydrazone schiffbase ligand.
The experimental results show conformably that the mode of binding of the complex fesal2trienno3 to dna is nonclassical electrostatic action, but. Apr 30, 2012 graphical abstract 2oxo1,2dihydrobenzohquinoline3carbaldehyde n ethylthiosemicarbazone and their pdii metal complexes has been synthesized and characterized. Mar 30, 2010 synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy benzoyl hydrazone. Synthesis and structural investigation of new biorelevant. They also can be served as complementary studies for the corresponding complexes of ruthenium. Syntheses of polypyridyl metal complexes and studies of their. Hemolytic and dna binding studies of divalent transition metal ion 531 of uvvisible spectra taken at room temperature. The necessity for cellular regulation of dna led to the development of metallonucleases to catalyze and repair dna strand breaks. This was done by using the hyperchem6 program for the molecular mechanics and. These studies revealed that the complexes showed an intercalative mode of binding to ct dna and also effectively cleaved the supercoiled. Silveri complexes with dna and rna studied by fourier. Metallointercalators with different metal centers, oxidation states, coordination geometries, and overall sizes will exhibit varying depths of insertion. The results of the cytotoxicity and dna cleavage studies are presented in this chapter. The molar conductance studies of cuii, niii, coii and mnii complexes showed nonelectrolyte in nature.
These aromatic side arms introduce new modes of dna binding, involving mutual interactions of functional groups held in close proximity. The complexes were screened for their antimicrobial activity. Electrochemical investigation and dnabinding studies of pefloxacinmetaliiiii complexes article pdf available in journal of coordination chemistry 6419. Jcprc5 1159 binding studies of some novel macroacyclic transition metal complexes towards ct dna via multispectroscopic techniques c. Synthesis, crystal structure, and dnabinding studies of a. Agi is a strong nucleic acids binder and forms several complexes with dna such as types i, ii, and iii. Design, syntheses and functional studies of dna binding. Synthesis, characterization, antimicrobial activity and. Binding of coii complex with calf thymus dna ct dna was studied by spectral methods. Jun 19, 2003 a series of mixed metal complexes coupling a lightabsorbing ruii terpyridine unit to a reactive cisptiicl2 moiety through polyazine bridging ligands have been prepared and their light absorbing, redox, and dna binding properties assayed. Hemolytic and dna binding studies of divalent transition.
Complexes of l1 were excellent for dna binding as compared to l2. Reactivity and structural studies of metal complexes bound. Truly, metal complexes bound to dna provide an interesting system to study structure, function and dynamics in a single package. Copperii complexes as potential anticancer and nonsteroidal. Pdf synthesis, characterization, dna binding interaction studies.
Bovine serum albumin interactions with metal complexes. Biological studies of copperii schiff base complexes. The cleavage efficiency of the complexes compared to that of the control is due to their efficient dna binding ability. Mic method was employed to inference the antibacterial potency of complexes in reference to free ligands and metal salt. The lack of molecular mechanistic understanding of the interaction between metal complexes and biomolecules hampers their potential medical use. Therefore, it was of interest to examine the binding. The tgdta study of the cobalt complex in air show that, it decomposes steadily to yield metal oxide as residue. Fluorescence scatchard plots demonstrated that the palladiumii complexes act as dualfunction metallointercalators and bind. In continuance of our ongoing research on dna binding and cleavage happenings of transition metal complexes, in this paper we obtainable the synthesis, characterization and dna cleavage. The general oxidative mechanisms proposed account of dna cleavage by hydroxyl radicals via. All the dna binding studies reveal that they are good intercalators. Dna binding properties of hl and complexes 14 have been investigated by.
Absorption spectral studies indicate that the coiii and niii complexes intercalate between the base pairs of the ctdna tightly with intrinsic dna binding constant of 1. For example, square planar complexes penetrate deeper into the dna. Synthesis, characterization and dnabinding studies of. Dna binding properties of copperii metal complex have been investigated by electronic absorption. Synthesis, characterization, antimicrobial, dna binding. Aminoantipyrine, schiff base, complex, antimicrobial studies, dna binding introduction. Synthesis, characterization, crystal structure and dna. Antimicrobial, anticancer and dna binding studies of. Dna cleavage, binding and intercalation studies of drug. In addition to these dna binding activity was also performed in order to elucidate the mode of binding the newly synthesised complexes.
Interactions of metal complexes with dna jihan talib university of wollongong talib, jihan, interactions of metal complexes with dna, phd thesis, school of chemistry. A series of mono and bimetallic metal complexes with cuii, ptii and znii with substituted polypyridyl ligands have been prepared and their binding affinities towards quadruplex cmyc and human telomeric and duplex dna ds26 and calf thymus determined using fluorescent indicator displacement fid assa metal interactions with nucleic acids. As a result, metallointercalator which contain an extended aromatic heterocyclic ligand can provide immensely powerful tools to probe nucleic acid 912. Synthesis, characterization, antimicrobial, dna binding and. Dna interactions and biocidal activity of metal complexes indian. Reactivity and structural studies of metal complexes bound to. Dna binding studies were studied by absorption spectra fluorescence. Synthesis, characterization, dna binding and cleavage of. We have also assessed the dna binding efficacy and mode of binding of both the complexes.
Hence, we report synthesis and characterization of novel schiff base ligand l and its complexes with cuii. Binding studies of 4, 5a, and 5b against calf thymus dna were carried out, revealing that 4, 5a, and 5b have. Stability, energetics and dna binding studies of zinc complexes. Electronic absorption spectroscopy serves as the most common means to study the interactions between metal complexes and dna barton et al. Synthesis, characterisation and dna binding studies of. Transition metal complexes have attracted curiosity due to dna binding and cleavage properties under physiological conditions. From all the spectral data a distorted octahedral geometry has proposed for both the complexes. Investigation of dna binding mechanism, photoinduced. Stability, energetics and dna binding studies of zinc. We hope the results will be of high value in further understanding of dna binding, the efficiency of dna recognition and cleavage by cuii complexes as well as laying the foundation for the rational design of new photoprobes and photonucleases. Post graduate and research department of chemistry, sri ramakrishna mission.
Synthesis, characterization and dna binding study of. In addition, the use of these metal complexes has allowed for observation of long range ct in dna mediated by dna binding proteins that contain redox sites iron sulfur cluster or disulfide linkages. Synthesis, characterization, dna binding interaction studies. Transition metal ions, mixed ligands, copper complexes, dna binding.
Prepared complexes showed enhanced dna binding tendency than the parent drug that might be of interest for future research. Dna binding, amelioration of oxidative stress, and molecular docking study of znii metal complex of a new schiff base ligand. Dna binding and antimicrobial studies of polymercopperii complexes containing 1,10phenanthroline and lphenylalanine ligands. Dnabinding and photocleavage studies of cobalt iii. The results of these studies showed that dna binding strengthened as the size of the unique ligand was increased. Coiii and niii complexes containing bioactive ligands. Biomolecular interaction, anticancer and antiangiogenic. Spectroscopic, microbial and dna binding affinities of.
The molecular docking scores of 4, 5a, and 5b with bdna suggest a similar trend in behavior to that observed in the dnabinding study. Metal complexes coii,cuii and znii ions of schiff base having played a central role in the development of coordination chemistry. Synthesis, characterization and dna binding study of mixed. Evaluation of dna binding and cytotoxic activity of a new. A theoretical treatment of the formation of complexes in the gas phase was studied, this was done using. Detailed dna binding studies of these two complexes, together with three previously reported metal salphen complexes, are presented. Direct determination of metal complexes interaction with. Synthesis, characterization, dna binding interaction. Transition metals play a more important role in drug design because of their biologically active metal ions and ligands 6.
The results suggest that both complexes bind to dna through intercalation and enantioselectively interact with ctdna. Dna binding studies of the complexes were investigated by uvvis, fluorescence, thermal melting and viscosity measurements. Synthesis, characterization, antimicrobial, dna binding and cleavage studies of schi. Synthesis, characterization and dnabinding studies of transition metal complexes with imidazole ligands scientific. The dna cleavage studies of metal complexes presented more protruding activity in the attendance of h 2 o 2 associated to that in the absence of h 2 o 2. Therefore, viscosity measurements were carried out to further clarify the interaction of metal complexes and dna. In addition, dna binding properties of the ligand and its metal complexes have been investigated by electronic.
Aug 27, 2016 dna binding ability of these complexes was studied on ct dna by using uvvis absorption, fluorescence and viscometry. Synthesis, characterization, antimicrobial and dna. Synthesis, characterization, dna binding properties, fluorescence studies and antioxidant activity of transition metal complexes with hesperetin2hydroxy benzoyl hydrazone. Fluorescence studies and antioxidant activity of transition. Synthesis, structural, dna binding and cleavage studies of cu. The metal complexes were characterized on the basis of various spectroscopic techniques like ir studies and elemental analysis while the geometry of complexes was octahedral which is confirmed by electronic spectra and thermogravimetric analysis. Dna cleavage ability of the complexes was examined on pbr322 dna by using gel electrophoresis method. Feb 01, 2017 read evaluation of dna protein interactions and cytotoxic studies of copperii complexes incorporated with n, n donor ligands and terpyridine ligand, international journal of biological macromolecules on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Synthesis, structural characterization, and quadruplex dna.
Studies on dna binding to metal complexes of sal 2trien. Dna binding studies were carried out by viscosity method. The characterization studies of all the metal complexes have been done with appropriate methods. All the metal complexes were screened for antibacterial activities.
Structural data of dna binding and molecular docking studies. Mar 27, 2019 the conformational changes in hsa upon metal complexes binding was observed by monitoring 3d fluorescence spectra of hsa alone and in the presence of metal complexes in 1. The dna binding interaction of the complexes was evaluated by photo physical studies and viscosity measurements. The solution of dna in the buffer gave a uv absorbance ratio a260a280 of about 1. These studies are necessary for the further comprehension of binding of transition metal complexes to dna. Experimental results indicated that complexes and cipro. These studies confirm that the complex 1 binds to ctdna through intercalation. However, no significant studies were made on the dna binding and cleavage. Apr 09, 20 competitive binding studies with ethidium bromide have shown that both complexes strongly bind to fsdna, and the apparent binding constant k app calculated from emission studies was 2.
The binding constants of complexes have been determined by the absorption titration spectrofluorimetric method. Fid and spr studies have shown that the complexes have a modest selectivity 1 order of magnitude for quadruplex, particula. Introduction the chemistry of dinucleating ligands has received considerable attention largely due to their catalytic and bioinorganic relevance. These studies are necessary for further comprehension of binding of transition metal complexes to dna. Keywords dna binding and cleavage studies, copper ii complexes dna binding studies, intercalative binding mode and oxidative cleavage studies i. Dna binding and cleavage studies of two palladiumii. Dna binding, in silico docking and in vitro biological screening of some transition metal complexes of schiff base ligand as potential blockers of cancer causing receptors, int. Synthesis, characterization, dna binding, dna cleavage and. Aug 11, 2017 the interactions of the synthesized chalcone and its metal complexes with dna were studied using steady. Studies on dna binding to metal complexes of sal 2 trien. In vitro study on dna binding of rutheniumii complexes with.
Materials and methods all the reagents and solvents used for the synthesis of ligand and the metal complexes were analar grade of. The photochemical dna cleavage of the complexes is also demonstrated. However, the details of the binding mode of silveri in the agpolynucleotides remains unknown. Thus through a series of experiments in this thesis, attempts have been made to correlate the structure and dynamics of metal complexes bound to dna. The effects of extending of coplanarity in a series of. All materials were purchased and used without further purification. The complexes show en hanced antimicrobial activities complexes with the free ligand. Synthesis, characterization, dna binding and cleavage.
Morphological studies were carried out by scanning electron microscopic method. Schiff base and its metal complexes were screened against the bacterial strains such as gram. Dna binding studies were also carried out for the synthesized complexes. Transition metal complexes derived from n, s bidentate ligands. Optical photophysical techniques are widely used to study the binding model of the ligand, metal complexes, and dna but not to give sufficient clues to support a binding model. Pdf synthesis, characterization, dna binding properties. Sep 03, 2009 we have also investigated the interaction between the complexes and a sequence of duplex dna to gain some insight into the selectivity of the compounds for quadruplex structures. The binding affinities of these complexes with calf thymus dna are also reported. Herein we present a robust procedure combining resonant xray emission spectroscopy and multiscale molecular dynamics simulations, which allows for straightforward elucidation of the precise interaction mechanism at the atomic level. The dna binding studies of the cobalt complex containing the ligand smbtu is reported. Human serum albumin hsa and transferrin tf binding studies. Pushpa latha department of chemistry, noorul islam university, kumaracoil, kkdist, tamil nadu, india abstract schiff base metal complexes of cefixime such as cucefbenz 2 cl 2. The ir spectra of the complexes are compared with those of the free ligands in order to determine the.
Investigation of antimicrobial, antioxidant, and dna binding studies. Binding of metallointercalators to dna is reversible and depends on the properties of the intercalating molecule. These experiments provide compelling evidence that such chargetransfer processes between proteins mediated by dna may be used in biology. Based on these reports, the synthesized copperii schiff base complexes were screened to know whether these complexes have any cytotoxic and dna binding activities. In recent years, binding studies of transition metal complexes have become very.
Also, bioactive metal complexes are useful biochemical tools for the detection of dna both in vitro and inside the cell, as dna probes 14, 15. The complexes fesal2trienno3 and cusal2trien have been synthesized and their interaction with calf thymus dna has been investigated for the first time using uv spectra, fluorescence spectra, thermal denaturation, and viscosity measurements. The chemicals include 4hydroxybenzaldehyde, urea, acetyl acetone, magnesium bromide, 4aminoantipyrine, dimethylsulphoxide transition metal chlorides. Design, structural characterizations, dna binding, dft calculations, molecular docking and biological studies. Synthesis, spectral, dna binding and cleavage properties of. Evaluation of dnaprotein interactions and cytotoxic studies. Pdf synthesis and characterization of metal complexes.
View enhanced pdf access article on wiley online library html view. Design, syntheses and functional studies of dna binding metal. The photochemical dna cleavage of the two complexes is also demonstrated. The complexes show enhanced antimicrobial activities complexes with the free ligand. The proposed dna binding mode supports the large enhancement in the relative.
Their stronger dna binding activity dba compared with free ligand suggested them as a good dna binder. Synthesis, characterization, dna binding and cleavage studies of. The dna binding and cleavage study of the copper complex containing the ligand is reported. Dna binding and photocleavage studies of cobaltiii. Synthesis, characterization and dna binding study of ruthentumii thiosemicarbazone complexes k. Synthesis, characterization, and dna binding properties of a. Synthesis, characterization, dna binding properties. Structural data of dna binding and molecular docking. All metal complexes showed significant nuclease activity in the presence of h 2 o 2.
Pdf synthesis and dna binding studies of niii, coii. Characteristic binding constant kb and binding mode of complexes with calf thymus dna ctdna were determined using absorption titration 0. Synthesis, characterization, dna binding and cleavage studies. Dna binding behaviors of these complexes with calf thymus dna ct dna have been investigated by absorption spectra, viscosity, and thermal denaturation methods.